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p2A7
p2A7
規(guī)格:
貨期:
編號(hào):B221551
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) p2A7
商品貨號(hào) B221551
Designations p2A7
Species Homo sapiens, human
Depositors MG Persico
Vector
Construct size (kb): 5.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 2.700000047683716
Tissue: placenta
Gene product: steroid sulfatase (microsomal)(steroid sulfatase, microsomal) [STS]
Insert Size (kb) 2.700
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Insert contains PstI (2), SstI (2), PvuII, and HindIII sites.
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7 doublet, HindIII--4.6, 0.95; BamHI--5.7; PstI--2.85, 1.65, 1.3; XhoI uncut.
The complete insert hybridizes with sequences on X and Y. A PvuII/EcoRI digest yields 2 fragments from the insert--1.7 and 1.0 kb. The larger hybridizes only to the X chromosome. The smaller recognizes all the same fragments as p2A7.
References

Mohandas TK, et al. Steroid sulfatase gene in XX males. Am. J. Hum. Genet. 46: 369-376, 1990. PubMed: 2301402

Ross MT, et al. Long-range physical mapping around the human steriod sulfatase locus. Genomics 6: 528-539, 1990. PubMed: 2328992

Ballabio A, et al. Isolation and characterization of a steroid sulfatase cDNA clone: genomic deletions in patients with X-chromosome-linked ichthyosis. Proc. Natl. Acad. Sci. USA 84: 4519-4523, 1987. PubMed: 3474618

Fraser N, et al. Identification of incomplete coding sequences for steroid sulphatase on the human Y chromosome: evidence for an ancestral pseudoautosomal gene?. Development 101: 127-137, 1987. PubMed: 3503710

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