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pAB366Ben
pAB366Ben
規(guī)格:
貨期:
編號(hào):B233285
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pAB366Ben
商品貨號(hào) B233285
Designations pAB366Ben
Depositors BM Tyler
Biosafety Level 1
Vector Information
Size (kb): 7.1999998092651370
Vector: pAB366Ben (plasmid)
Construction: pAB366, pBT6
Marker(s):ampR,bleR,benR
Construct size (kb): 7.199999809265137
Features: marker(s): ampR, bleR, benR
replicon: pMB1
Applications
contains sequence tubulin, beta
in another host, produces protein tubulin, beta
reporter construct
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.1, 2.1; HindIII--4.0, 3.2; BamHI/HindIII--2.6, 2.5, 1.5, 0.65; BamHI/XhoI--3.0, 1.35, 1.2, 0.9, 0.8; SalI/HindIII--3.3, 2.6, 0.85, 0.55.
A 2.5 kb SalI/HindIII fragment contains the complete tubulin gene and 5' and 3' flanking regions.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Austin B, et al. Optimized vectors and selection for transformation of Neurospora crassa and Aspergillus nidulans to bleomycin and phleomycin resistance. Gene 93: 157-162, 1990. PubMed: 1699844

Orbach MJ, et al. Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker. Mol. Cell. Biol. 6: 2452-2461, 1986. PubMed: 2946938

Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151

Shipped frozen
Shipping Information ?Frozen glycerol stock of Escherichia coli containing the plasmid.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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