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pCNV220
pCNV220
規(guī)格:
貨期:
編號:B233667
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pCNV220
商品貨號 B233667
Designations pCNV220
GenBank Number

M25270

Species Cucumber necrosis virus (CNV)
Depositors DM Rochon
Applications
contains sequence genome, 90%+ complete
Vector
Construct size (kb): 7.5
Insert
DNA: cDNA
Insert lengths(kb): 4.699999809265137
Gene product: genome, 90%+ complete
Target Gene: genome, 90%+ complete
Insert Size (kb) 4.700
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.6, 3.0; HindIII--3.5, 1.4, 1.3, 1.2; BglI--2.7, 1.7, 1.5 (doublet).
Insert contains the following restriction sites (approximate kb from the 5' end): BglI--2.66, 4.29; BglII--3.36; EcoRI--2.14; EcoRV--1.07, 1.49, 1.57, 3.19, 4.03; HindIII--0.62, 1.80, 3.22; KpnI--3.89; NdeI--0.64, 1.58, 3.52, 3.63; PvuII--2.49, 3.33.
Contains entire coding sequence plus 20 nucleotides of 5' noncoding sequence.
Virus was isolated from cucumber, Cucumis sativus (cultivar strain 8).
Classification Tombusvirus
References

Rochon DM, Tremaine JH. Complete nucleotide sequence of the cucumber necrosis virus genome. Virology 169: 251-259, 1989. PubMed: 2705296

D'Ann M Rochon, personal communication

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